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multiscribe high-cdna capacity rt kits  (Thermo Fisher)


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    Structured Review

    Thermo Fisher multiscribe high-cdna capacity rt kits
    Multiscribe High Cdna Capacity Rt Kits, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multiscribe+rt+kit/multiscribe+high+cdna+capacity+rt+kits/pm40426130-494-8-13
    Average 90 stars, based on 1 article reviews
    multiscribe high-cdna capacity rt kits - by Bioz Stars, 2026-11
    90/100 stars

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    Reverse Transcription:

    Article Title: LINC00052 Promotes Gastric Cancer Cell Proliferation and Metastasis via Activating the Wnt/β-Catenin Signaling Pathway
    Article Snippet: Total RNA was isolated from GC cell lines and GC tissue samples using TRIzol reagent (Invitrogen) and treated with DNaseI (Promega, Madison, WI, USA). .. Reverse transcription was performed using the Multiscribe RT Kit (Applied Biosystems, Foster City, CA, USA) and random hexamers or oligo(dT). .. qRT-PCR was performed by 10 ng of cDNA, 5 μl of SYBR Green PCR Master Mix (Applied Biosystems), and 200 nM of each gene-specific primer.

    Article Title: 2'-Fucosyllactose promotes Lactobacillus rhamnosus KLDS 8001 to repair LPS-induced damage in Caco-2 cells.
    Article Snippet: Human milk oligosaccharides (HMOs) are the secondlargest carbohydrate component and the third most nutritious component of mature breast milk, with a content of about 10– 15 g/L (Thum et al., 2021).. At present, more than 200 HMOs have been identified, among which the representative 2′FL is the most abundant, accounting for about 30% of the total (Plows et al., 2021).. HMOs, serve an essential function in sustaining the intestinal microecological health of infants as a nondigestible source of natural prebiotics (Sanchez et al., 2021).

    Article Title: Arenobufagin increases the sensitivity of gastric cancer to cisplatin via alkaliptosis
    Article Snippet: Fluorescent signals were detected by confocal uorescence microscopy. qRT-PCR Total RNA was isolated from AGS cells and MKN-45 cells as well as tumorigenic tissues of nude mice using TRIzol reagent (Invitrogen) according to manufacturer’s instructions. .. Reverse transcription was performed by the Multiscribe RT kit (Applied Biosystems, Foster, CA, USA). .. For analysis of CA9, NF-κB, IKBKB, MMP-2, MMP-9 and E-cadherin, the SYBR Green PCR kit (TaKaRa, Japan) was used to quantify the messenger RNA (mRNA) levels.

    Article Title: Procaine Inhibits the Proliferation and Migration of Colon Cancer Cells Through Inactivation of the ERK/MAPK/FAK Pathways by Regulation of RhoA
    Article Snippet: Total RNA was isolated from transfected cells using TRIzol reagent (Invitrogen) and treated with DNaseI (Promega, Madison, WI, USA). .. Reverse transcription was performed using the MultiScribe RT Kit (Applied Biosystems, Foster City, CA, USA) and random hexamers or oligo (dT). ..

    Article Title: Arenobufagin increases the sensitivity of gastric cancer to cisplatin via alkaliptosis
    Article Snippet: Total RNA was isolated from the tumorigenic tissues of nude mice using TRIzol reagent (Invitrogen) according to the manufacturer's instructions. .. Reverse transcription was performed using a MultiScribe RT kit (Applied Biosystems, Foster City, CA, USA). .. For analysis of CA9, NF-κB, IKBKB, MMP-2, MMP-9, and E-cadherin, the SYBR Green PCR kit (TaKaRa, Japan) was used to quantify the messenger RNA (mRNA) levels.

    Article Title: Obesity Is Associated with Distorted Proteoglycan Expression in Adipose Tissue
    Article Snippet: The upper phase was mixed with alcohol and loaded onto Nucleospin RNA purification columns (Macherey-Nagel, Düren, Germany) to purify total RNA. .. Total RNA was reverse transcribed into cDNA using Multiscribe ® RT kit (Thermo Fisher, Waltham, MA, USA). .. RT-qPCR was performed with the Bio-Rad SsoAdvancedTM Universal SYBR ® Green Supermix using 5–10 ng/μL cDNA.

    cDNA Synthesis:

    Article Title: Cimifugin Alleviates Chronic Constriction Injury of the Sciatic Nerve by Suppressing Inflammatory Response and Schwann Cell Apoptosis.
    Article Snippet: Inflammation and Schwann cell apoptosis play critical roles in neuropathic pain after sciatic nerve injury.. This study aimed to explore the function and mechanism of cimifugin in lipopolysaccharide (LPS)-stimulated rat Schwann cells and sciatic nerves of rats treated with chronic constriction injury (CCI).. Thermal, mechanical and cold hyperalgesia of rats in response to cimifugin or mecobalamin (the positive drug control) treatment were evaluated through behavioral tests.



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